Karlstein
Karlstein may refer to places in:
- Germany
- Karlstein am Main, a municipality in Landkreis Aschaffenburg, Bavaria
- part of Bad Reichenhall, Bavaria - a rock with a ruin there
 
- Karlstein bei Hornberg, a rock in Hornberg, Baden-Württemberg
- part of Regenstauf, Landkreis Regensburg, Bavaria - Schloss Karlstein, the castle there
 
- Aussichtspunkt Karlstein, a site above Weinstadt, Baden-Württemberg
- Austria
- Karlstein an der Thaya, a municipality in Lower Austria, Austria - Schloss Karlstein, the castle there
- Internierungslager Karlstein an der Thaya, a former internment camp there
 
- Czech Republic
- Karlštejn Castle, a 14th-century castle in Central Bohemia - Karlštejn (Beroun District), městys named after the castle
- Karlštejn, nature reserve nearby the castle
 
- Hunting castle Karlštejn nearby village Svratouch in Pardubice Region (Chrudim District) - Karlštejn, administrative part of village Svratouch, named after the hunting castle
- Karlštejn, hill in Žďárské vrchy where the hunting castle is situated