| Chapter | Charter date and range | Institution | Location | Status | References |
| Alpha | 1921 | A.T. Still University | Kirksville, Missouri | Active | [4][a] |
| Beta | 1925 | Des Moines University | Des Moines, Iowa | Active | [4][b] |
| Gamma (First) | 1930 | Los Angeles College of Osteopathic Physicians and Surgeons | Irvine, California | Inactive, Reassigned | [4][c] |
| Delta | 1932 | Kansas City University | Kansas City, Missouri | Active | [4][d] |
| May 2018 | Kansas City University, Joplin | Joplin, Missouri |
| Epsilon | 1939 | Chicago College of Osteopathic Medicine | Downers Grove, Illinois | Active | [4][5] |
| Zeta | 19xx ?–19xx ?; March 1997 | Philadelphia College of Osteopathic Medicine | Philadelphia, Pennsylvania | Active | |
| | Philadelphia College of Osteopathic Medicine, Moultrie Campus | Moultrie, Georgia |
| | Philadelphia College of Osteopathic Medicine, Suwanee Campus | Suwanee, Georgia |
| Eta | | University of North Texas Health Science Center | Fort Worth, Texas | Active | |
| Theta | | Oklahoma State University Center for Health Sciences College of Osteopathic Medicine | Tulsa, Oklahoma | Active | |
| Iota | | Michigan State University College of Osteopathic Medicine | East Lansing, Michigan | Active | |
| Kappa | | University of New England College of Osteopathic Medicine | Biddeford, Maine | Active | |
| Lambda | | Heritage College of Osteopathic Medicine | Athens, Ohio | Active | [6] |
| September 2016 | Heritage College of Osteopathic Medicine, Dublin | Dublin, Ohio |
| September 2019 | Heritage College of Osteopathic Medicine, Cleveland | Cleveland, Ohio |
| Mu | | College of Osteopathic Medicine of the Pacific | Pomona, California | Active | |
| | College of Osteopathic Medicine of the Pacific, Northwest | Lebanon, Oregon |
| Nu | | West Virginia School of Osteopathic Medicine | Lewisburg, West Virginia | Active | [7] |
| Xi | | Nova Southeastern College of Osteopathic Medicine | Davie, Florida | Active | |
| Nova Southeastern College of Osteopathic Medicine, Clearwater | Clearwater, Florida |
| Gamma (Second) | May 8, 2002 | Lake Erie College of Osteopathic Medicine | Erie, Pennsylvania | Active | [8][9] |
| February 2012 | Lake Erie College of Osteopathic Medicine at Bradenton | Bradenton, Florida |
| October 2016 | Lake Erie College of Osteopathic Medicine at Seton Hill | Greensburg, Pennsylvania |
| | Lake Erie College of Osteopathic Medicine at Elmira | Elmira, New York |
| Omicron | October 12, 2003 | Rowan-Virtua School of Osteopathic Medicine | Stratford, New Jersey | Active | [e] |
| Pi | | Touro University College of Osteopathic Medicine, Nevada | Henderson, Nevada | Inactive | |
| Rho | | Arizona College of Osteopathic Medicine | Glendale, Arizona | Active | |
| Sigma | October 12, 2003 | University of Pikeville Kentucky College of Osteopathic Medicine | Pikesville, Kentucky | Active | |
| Tau | October 2006 | Touro University California College of Osteopathic Medicine | Vallejo, California | Active | |
| October 2009 | Touro University California College of Osteopathic Medicine, Henderson | Henderson, Nevada |
| Upsilon | | A.T. Still University: School of Osteopathic Medicine in Arizona | Mesa, Arizona | Active | |
| Phi | February 2008 | Lincoln Memorial University - DeBusk College of Osteopathic Medicine | Harrogate, Tennessee | Active | |
| Chi | October 2006 | Edward Via College of Osteopathic Medicine | Blacksburg, Virginia | Active | |
| October 2012 | Edward Via College of Osteopathic Medicine, Carolinas Campus | Spartanburg, South Carolina |
| Fall 2015 | Edward Via College of Osteopathic Medicine, Auburn Campus | Auburn, Alabama |
| Psi | | Touro University College of Osteopathic Medicine, New York | Harlem, New York City, New York | Active | [10] |
| February 2017 | Touro University College of Osteopathic Medicine, Middletown | Middletown, New York |
| | Touro University College of Osteopathic Medicine, Great Falls | Great Falls, Montana |
| Omega | 19xx ?–xxxx ?; February 16, 2008 | New York Institute of Technology College of Osteopathic Medicine | Old Wesbury, New York | Active | |
| Spring 2019 | New York Institute of Technology College of Osteopathic Medicine, Jonesboro Campus | Jonesboro, Arkansas |
| Alpha Alpha | | Lake Erie College of Osteopathic Medicine, Bradenton Campus | Bradenton, Florida | Inactive | |
| Gamma Delta | April 2011 | Pacific Northwest University College of Osteopathic Medicine | Yakima, Washington | Active | |
| Omicron Theta | September 2011 | Rocky Vista University College of Osteopathic Medicine | Englewood, Colorado | Active | |
| September 2019 | Rocky Vista University College of Osteopathic Medicine, Ivins Campus | Ivins, Utah |
| Alpha Mu | October 2012 | William Carey University College of Osteopathic Medicine | Hattiesburg, Mississippi | Active | |
| Mu Upsilon | December 2014 | Marian University College of Osteopathic Medicine | Indianapolis, Indiana | Active | [11] |
| Alpha Omega | February 2015 | Liberty University College of Osteopathic Medicine | Lynchburg, Virginia | Active | |
| Alpha Beta | June 2015 | Alabama College of Osteopathic Medicine | Dothan, Alabama | Active | |
| Alpha Gamma | | College of Osteopathic Medicine, Sam Houston State University | Huntsville, Texas | Active | |
| Alpha Lambda | | California Health Sciences University College of Osteopathic Medicine | Clovis, California | Active | |
| Chi Upsilon | August 2017 | Campbell University Jerry M Wallace School of Osteopathic Medicine | Lillington, North Carolina | Active | |
| Beta Chi | November 2017 | Burrell College of Osteopathic Medicine | Las Cruces, New Mexico | Active | [12][13] |
| | Burrell College of Osteopathic Medicine, Melbourne | Melbourne, Florida |
| Alpha Rho | September 2018 | Arkansas College of Osteopathic Medicine | Fort Smith, Arkansas | Active | |
| Alpha Psi | | University of the Incarnate Word School of Osteopathic Medicine | San Antonio, Texas | Active | |
| Zeta Alpha | | Philadelphia College of Osteopathic Medicine, Georgia Campus | Suwanee, Georgia | | |
| Iota Delta | | Idaho College of Osteopathic Medicine | Meridian, Idaho | Active | |
| Kappa Chi | 202x ? | Kansas College of Osteopathic Medicine | Wichita, Kansas | Active | |