2010 Copa Sudamericana preliminary stages
The preliminary stages of the 2010 Copa Sudamericana de Clubes are:
- First Stage, contested by 16 teams in 8 two-legged ties. The winner of each tie will advance to the Second Stage.
 - Second Stage, contested by 30 teams, 8 of whom advanced from the First Stage, in 15 two-legged ties. The winner of each tie will advance to the Round of 16, joining the defending champion, LDU Quito.
 
First stage
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg | 
|---|---|---|---|---|
| Colo-Colo  |  3–3 (a) | 0–2 | 3–1 | |
| River Plate  |  4–4 (a) | 0–2 | 4–2 | |
| Barcelona  |  5–2 | 2–1 | 3–1 | |
| Trujillanos  |  2–5 | 1–4 | 1–1 | |
| Oriente Petrolero  |  3–2 | 2–2 | 1–0 | |
| Olimpia  |  1–3 | 0–2 | 1–1 | |
| Universidad San Martín  |  (a) 3–3 | 2–3 | 2–1 | |
| Santa Fe  |  4–2 | 0–2 | 4–0 | 
 
Match A
| Universitario  | 2–0 | |
|---|---|---|
| Galindo  Lima  |  Report | 
| Colo-Colo  | 3–1 | |
|---|---|---|
| Cámpora  Fuenzalida  |  Report | Galindo  |  
Colo-Colo 3–3 Universitario on points. Universitario advanced on away goals.
Match B
| Guaraní  | 2–0 | |
|---|---|---|
| Fabbro  Teixeira  |  Report | 
| River Plate  | 4–2 | |
|---|---|---|
| Ramírez  Zambrana Correa  |  Report | Teixeira  Joel Benítez  |  
River Plate 3–3 Guaraní on points. Guaraní advanced on away goals.
Match C
| Universidad César Vallejo  | 1–2 | |
|---|---|---|
| H. Hurtado  |  Report | J. Hurtado  Noir  |  
| Barcelona  | 3–1 | |
|---|---|---|
| Noir  Oyola Samudio  |  Report | Cazulo  |  
Barcelona advanced on points 6–0.
Match D
| Atlético Huila  | 4–1 | |
|---|---|---|
| Carbonero  Castillo Martínez Velásquez  |  Report | R. García  |  
| Trujillanos  | 1–1 | |
|---|---|---|
| R. García  |  Report | Rueda  |  
Atlético Huila advanced on points 4–1.
Match E
| Universidad de Chile  | 2–2 | |
|---|---|---|
| Bueno  Puch  |  Report | Rea  Saucedo  |  
Oriente Petrolero advanced on points 4–1.
Match F
| Defensor Sporting  | 2–0 | |
|---|---|---|
| Mora  |  Report | 
| Olimpia  | 1–1 | |
|---|---|---|
| Ferreyra  |  Report | Mora  |  
Defensor Sporting advanced on points 4–1.
Match G
| Deportivo Quito  | 3–2 | |
|---|---|---|
| Mina  Fernández Checa  |  Report | Vitti  Alemanno  |  
| Universidad San Martín  | 2–1 | |
|---|---|---|
| Arriola  Alemanno  |  Report | Checa  |  
Universidad San Martín 3–3 Deportivo Quito on points. Universidad San Martín advanced on away goals.
Match H
| Deportivo Lara  | 2–0 | |
|---|---|---|
| Ocanto  Chalar  |  Report | 
| Santa Fe  | 4–0 | |
|---|---|---|
| Otálvaro  Bernal Noguera Anchico  |  Report | 
Santa Fe 3–3 Deportivo Lara on points. Santa Fe advanced on goal difference.
Second stage
| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg | 
|---|---|---|---|---|
| San José  |  5–1 | 1–1 | 4–0 | |
| Argentinos Juniors  |  1–2 | 0–1 | 1–1 | |
| Peñarol  |  3–1 | 1–0 | 2–1 | |
| Palmeiras  |  3–2 | 0–2 | 3–0 | |
| Caracas  |  1–2 | 1–2 | 0–0 | |
| Avaí  |  3–2 | 3–1 | 0–1 | |
| Deportes Tolima  |  2–1 | 0–1 | 2–0 | |
| Unión San Felipe  |  2–2 (8–7 p) | 1–1 | 1–1 | |
| Banfield  |  2–1 | 1–0 | 1–1 | |
| Emelec  |  6–2 | 1–2 | 5–0 | |
| Atlético Mineiro  |  1–0 | 0–0 | 1–0 | |
| Cerro Porteño  |  2–3 | 0–1 | 2–2 | |
| Grêmio  |  1–3 | 1–1 | 0–2 | |
| Sport Huancayo  |  2–9 | 0–9 | 2–0 | |
| Estudiantes  |  1–2 | 0–1 | 1–1 | 
Match O1
| Atlético Huila  | 1–1 | |
|---|---|---|
| Martínez  |  Report | de Souza  |  
| San José  | 4–0 | |
|---|---|---|
| Villalba  de Souza Bejarano Medina  |  Report | 
San José advanced on points 4-1.
Match O2
| Argentinos Juniors  | 1–1 | |
|---|---|---|
| Ortigoza  |  Report | Gracián  |  
Independiente advanced on points 4–1.
Match O3
| Peñarol  | 2–1 | |
|---|---|---|
| Ramis  Pacheco  |  Report | S. Sosa  |  
Peñarol advanced on points 6–0.
Match O4
| Palmeiras  | 3–0 | |
|---|---|---|
| Tadeu  Marcos Assunção  |  Report | 
Palmeiras 3–3 Vitória on points. Palmeiras advanced on goal difference.
Match O5
| Caracas  | 0–0 | |
|---|---|---|
| Report | 
Santa Fe advanced on points 4–1.
Match O6
| Avaí  | 0–1 | |
|---|---|---|
| Report | Zé Eduardo  |  
Avaí 3–3 Santos on points. Avaí advanced on goal difference.
Match O7
| Deportes Tolima  | 2–0 | |
|---|---|---|
| Medina  Perlaza  |  Report | 
Deportes Tolima 3–3 Oriente Petrolero on points. Deportes Tolima advanced on goal difference.
Match O9
| Guaraní  | 1–1 | |
|---|---|---|
| Ortiz  |  Report | Vildozo  |  
| Unión San Felipe  | 1–1 | |
|---|---|---|
| González  |  Report | Carballo  |  
| Penalties | ||
| González  Páez Suárez Vildozo Pérez Fernández Quiñones Bravo Domínguez Estay Magaña  |  8–7 | |
Unión San Felipe 3–3 Guaraní on points. Unión San Felipe advanced 8–7 on penalties.
Match O10
| Vélez Sársfield  | 0–1 | |
|---|---|---|
| Report | García  |  
Banfield advanced on points 4–1.
Match O11
| Universidad San Martín  | 2–1 | |
|---|---|---|
| Vitti  Quinteros  |  Report | Rojas  |  
| Emelec  | 5–0 | |
|---|---|---|
| Ayoví  Quiroz Giménez Valencia  |  Report | 
Emelec 3–3 Universidad San Martín on points. Emelec advanced on goal difference.
Match O12
| Grêmio Prudente  | 0–0 | |
|---|---|---|
| Report | 
| Atlético Mineiro  | 1–0 | |
|---|---|---|
| Ricardinho  |  Report | 
Atlético Mineiro advanced on points 4–1.
Match O13
| Universitario  | 1–0 | |
|---|---|---|
| Galindo  |  Report | 
| Cerro Porteño  | 2–2 | |
|---|---|---|
| dos Santos  Núñez  |  Report | Galindo  Cirillo  |  
Universitario advanced on points 4–1.
Match O14
| Grêmio  | 0–2 | |
|---|---|---|
| Report | Amaral  Éverton Santos  |  
Goiás advanced on points 4–1.
Match O15
| Defensor Sporting  | 9–0 | |
|---|---|---|
| de Souza  Risso Amado Mora Luna Aranda  |  Report | 
Note: This is the largest margin of victory in the history of the Copa Sudamericana.
| Sport Huancayo  | 2–0 | |
|---|---|---|
| Suárez  Ramírez  |  Report | 
Sport Huancayo 3–3 Defensor Sporting on points. Defensor Sporting advanced on goal difference.
Match O16
| Newell's Old Boys  | 1–0 | |
|---|---|---|
| Formica  |  Report | 
| Estudiantes  | 1–1 | |
|---|---|---|
| Fernández  |  Report | Borghello  |  
Newell's Old Boys advanced on points 4–1.