| Name | Location | Operator | Herd size |
| American Prairie | Montana | American Prairie Foundation | 800+ |
| Antelope Island State Park[3] | Utah | Utah State Parks | 200 |
| Badlands National Park [3] | South Dakota | National Park Service | 1000 |
| Banff National Park[3] | Alberta | Parks Canada | 36 |
| Battelle Darby Creek Metro Park | Ohio | Columbus and Franklin County Metro Parks | 10 |
| Bear Butte State Park | South Dakota | South Dakota Department of Game, Fish, and Parks | small |
| Bear River State Park[3] | Wyoming | Wyoming Division of State Parks and Historic Sites | 10 |
| Blue Mounds State Park[3] | Minnesota | Minnesota Department of Natural Resources | 100 |
| Broken Kettle Grasslands Preserve | Iowa | The Nature Conservancy | 200 |
| Buffalo Pound Provincial Park[3] | Saskatchewan | Saskatchewan Environment, Parks Branch | |
| Camp Pendleton bison herd | California | USMC (Camp Pendleton Game Warden's Office) | 90 |
| Camp Wainwright[3] | Alberta | Department of National Defence (Canada) | |
| Caprock Canyons State Park[3] | Texas | Texas Parks and Wildlife Department | 150 |
| Catalina Island bison herd[3] | California | Catalina Island Conservancy | 100 |
| Chickasaw National Recreation Area | Oklahoma | National Park Service | 3 |
| Clymer Meadow Preserve[3] | Texas | The Nature Conservancy | |
| Chief Mountain Wilderness Unit[4] | Montana | Blackfeet Nation | 25 |
| Chippewa Cree Tribal Buffalo Pasture | Montana | Chippewa Cree | 11 |
| Chitina herd[3] | Alaska | Alaska Department of Fish and Game | |
| Copper River herd[3] | Alaska | Alaska Department of Fish and Game | |
| Crane Trust | Nebraska | Crane Trust | 50+ |
| Cross Ranch Nature Preserve[3] | North Dakota | The Nature Conservancy | 200 |
| CSKT Bison Range | Montana | Confederated Salish and Kootenai Tribes | 500 |
| Custer State Park[3] | South Dakota | South Dakota Department of Game, Fish, and Parks | 1500 |
| Daniels Park[3] | Colorado | Denver Parks and Recreation | 30 |
| Delta Junction herd[3] | Alaska | Alaska Department of Fish and Game | |
| Elk Island National Park[3] | Alberta | Parks Canada | 315 |
| Farewell Lake herd[3] | Alaska | Alaska Department of Fish and Game | |
| Fermilab bison herd[3] | Illinois | U.S. Department of Energy | 28 |
| Finney Game Refuge[3] | Kansas | Kansas Department of Wildlife and Parks | |
| Fort Niobrara National Wildlife Refuge[3] | Nebraska | U.S. Fish and Wildlife Service | 350 |
| Fort Peck Indian Reservation | Montana | Assiniboine and Sioux Tribes | 400 |
| Fort Robinson State Park[3] | Nebraska | Nebraska Game and Parks Commission | 150 |
| Fort Worth Nature Center and Refuge | Texas | Fort Worth, Texas | 18 |
| Genesee Park[3] | Colorado | Denver Parks and Recreation | 33 |
| Grand Teton National Park–National Elk Refuge bison herd[3] | Wyoming | National Park Service | 1000 |
| Grasslands National Park | Saskatchewan | Parks Canada | 300 |
| Hay-Zama Lakes Wildland Park | Alberta | Alberta Parks | 400 |
| Henry Mountains bison herd[3] | Utah | Utah Division of Wildlife Resources, Bureau of Land Management | 400 |
| Hot Springs State Park[3] | Wyoming | Wyoming Division of State Parks and Historic Sites | 15 |
| House Rock State Wildlife Area[3] | Arizona | Arizona Game and Fish Department | |
| Innoko National Wildlife Refuge | Alaska | U.S. Fish and Wildlife Service | 94 |
| Janos Biosphere Reserve | Chihuahua | Secretariat of Environment and Natural Resources | 138 |
| Kankakee Sands | Indiana | The Nature Conservancy | 90+ |
| Konza Prairie Biological Station[3] | Kansas | The Nature Conservancy, Kansas State University | 300 |
| Land Between the Lakes National Recreation Area[3] | Kentucky | U.S. Forest Service | 50 |
| Maderas del Carmen | Coahuila | Secretariat of Environment and Natural Resources | 19 |
| Maxwell Wildlife Refuge | Kansas | Kansas Department of Wildlife and Parks | 150 |
| Medano–Zapata Ranch | Colorado | The Nature Conservancy | |
| Midewin National Tallgrass Prairie | Illinois | U.S. Forest Service | 50 |
| Minneopa State Park | Minnesota | Minnesota Department of Natural Resources | 30 |
| Nachusa Grasslands | Illinois | The Nature Conservancy | 130 |
| Niobrara Valley Preserve | Nebraska | The Nature Conservancy | |
| Neal Smith National Wildlife Refuge[3] | Iowa | U.S. Fish and Wildlife Service | 50 |
| Old Man on His Back Prairie and Heritage Conservation Area | Saskatchewan | Nature Conservancy of Canada | 115 |
| Ordway Prairie Preserve | South Dakota | The Nature Conservancy | |
| Paynes Prairie Preserve State Park | Florida | Florida Department of Environmental Protection | 50-70 |
| Pink Mountain[3] | British Columbia | British Columbia Department of Water, Lands and Air Protection | |
| Prairie State Park[3] | Missouri | Missouri Department of Natural Resources | 100 |
| Primrose Lake Air Weapons Range[3] | Alberta–Saskatchewan | CFB Cold Lake, Department of National Defence (Canada) | |
| Prince Albert National Park[3] | Saskatchewan | Parks Canada | 400 |
| Raymond State Wildlife Area[3] | Arizona | Arizona Game and Fish Department | |
| Riding Mountain National Park[3] | Manitoba | Parks Canada | 40 |
| Rocky Mountain Arsenal National Wildlife Refuge | Colorado | U.S. Fish and Wildlife Service | 150 |
| Sandhill Wildlife Area[3] | Wisconsin | Wisconsin Department of Natural Resources | 15 |
| Sandsage Bison Range & Wildlife Area | Kansas | Kansas Department of Wildlife and Parks | 51 |
| Smoky Valley Ranch | Kansas | The Nature Conservancy | |
| Soapstone Prairie/Red Mountain | Colorado | Fort Collins, Colorado and Larimer County | 100 |
| Spring Lake Park Reserve | Minnesota | Dakota County Parks | 12 |
| Tallgrass Prairie National Preserve | Kansas | The Nature Conservancy, National Park Service | 90 |
| Tallgrass Prairie Preserve[3] | Oklahoma | The Nature Conservancy | 2500 |
| Theodore Roosevelt National Park[3] | North Dakota | National Park Service | 800 |
| Trexler Nature Preserve | Pennsylvania | Lehigh County | 14 |
| Vermejo Park Ranch | New Mexico and Colorado | Ted Turner | 1200 |
| Wanuskewin Heritage Park | Saskatchewan | Wanuskewin Heritage Park Authority | 12 |
| Waterton Lakes National Park[3] | Alberta | Parks Canada | 6 |
| White Horse Hill National Game Preserve[3] | North Dakota | U.S. Fish and Wildlife Service | 20 |
| Wichita Mountains Wildlife Refuge[3] | Oklahoma | U.S. Fish and Wildlife Service | 650 |
| Wildcat Hills State Recreation Area[3] | Nebraska | Nebraska Game and Parks Commission | |
| Wind Cave National Park | South Dakota | National Park Service | 400 |
| Wolakota Buffalo Range | South Dakota | Rosebud Economic Development Corporation | 100 |
| Wood Buffalo National Park | Alberta and Northwest Territories | Parks Canada | 3000 |
| Yellowstone National Park | Idaho, Montana, Wyoming | National Park Service | 4800 |
| Commercial herds are not included. Listed herds remove excess bison on a regular basis which may be slaughtered for commercial purposes. |