| Player | Position | Seasons | Notes | Ref |
| Lady Baldwin | P | 1890 | Baldwin appeared in 7 games for Buffalo in 1890, going 2-5. He retired after the season. | [1] |
| Ed Beecher | OF | 1890 | Beecher appeared in 126 games, collecting 159 hits, 90 RBI, and a .297 batting average. He led the team in hits and at bats. | [2] |
| John Buckley | P | 1890 | Buckley pitched in 4 games for Buffalo, going 1-3 with a 7.68 ERA. 1890 was his only season in the major leagues. | [3] |
| Jack Carney | 1B / OF | 1890 | Carney split the 1890 season with the Cleveland Infants. In his 28 games for the Bisons, Carney collected 29 hits in 107 at bats, hitting for a .271 batting average. | [4] |
| Spider Clark | P | 1890 | Clark appeared in 69 games for the Bisons in 1890. He hit .265 with Buffalo, in his second and final season in the majors. | [5] |
| Dan Cotter | P | 1890 | Cotter pitched for the Bisons in the only game of his career, throwing a complete game loss, giving up 18 hits and 14 earned runs. | [6] |
| Bert Cunningham | P | 1890 | Cunningham split the 1890 season with the Bisons and the Philadelphia Athletics. He, along with George Haddock, led Buffalo with 9 wins, and he had the only 2 shutouts for Buffalo. | [7] |
| Fred Doe | SP | 1890 | Doe pitched in 1 game for both the Bisons and the Pittsburgh Burghers in 1890. He got his only decision, a loss, for Buffalo. | [8] |
| Bill Duzen | SP | 1890 | Duzen, who pitched only in 1890, loss both of the games he pitched, giving up 20 earned runs in 13.0 innings pitched. | [9] |
| Jay Faatz* | 1B | 1890 | In 1890, Faatz played in 32 games for the Bisons, hitting .189 and driving in 16 RBI. | [10] |
| Alex Ferson | SP | 1890 | Ferson had a 1-7 win–loss record in 10 games for Buffalo, with a 5.45 ERA. | [11] |
| Jim Gillespie | OF | 1890 | Gillespie played in his only game in the major leagues for Buffalo, going 0-3, with 2 strikeouts | [12] |
| George Haddock | SP | 1890 | Haddock led the Bisons, along with Bert Cunningham in wins, with 9. He also led the Players' League in losses, with 26. | [13] |
| Jocko Halligan | OF | 1890 | Halligan hit .251 in 57 games for the Bisons. | [14] |
| Dummy Hoy | CF | 1890 | Hoy appeared in 122 games for Buffalo, hitting for a .298 batting average. Hoy led the Bisons in runs, with 107, and stolen bases, with 39. | [15] |
| John Irwin | 3B | 1890 | Marr played in 77 games for Buffalo, having 31 hits, and a .234 batting average | [16] |
| George Keefe | SP | 1890 | Keefe had a 6-16 record in 25 games for the Bisons. | [17] |
| Gus Krock | SP | 1890 | In 4 games, Krock had a 0-3 record, with a 6.12 earned run average. 1890 was Krocks' final season in the majors. | [18] |
| Lewis | P | 1890 | Lewis (whose first name is unknown) pitched in 3 innings in his only game in the majors, getting the loss. He also had 1 hit in 5 at bats. | [19] |
| Connie Mack† | C | 1890 | In 123 games, Mack went 134 for 503, with a .266 batting average. He collected a career high 53 RBI. | [20] |
| John Rainey | OF | 1890 | Rainey had a .235 batting average in 42 games for Buffalo. | [21] |
| Jack Rowe* | SS | 1890 | Rowe had 126 hits for the Bisons in 125 games. 1890 was Rowe's final major league season. | [22] |
| General Stafford | SP / OF | 1890 | Stafford appeared for the Bisons as both a pitcher and an outfielder. He hit for a .143 batting average, in 49 at bats. Stafford also had a 3-9 win–loss record, with a 5.14 ERA. | [23] |
| Larry Twitchell | OF / P | 1890 | Twitchell was both an outfielder and a pitcher for the Bisons, hitting .221, and going 5-7 in 12 games started. | [24] |
| Deacon White† | 3B / 1B | 1890 | White, in his final major league season, played in 122 games for Buffalo in 1890. He drove in 47 runs and had 114 hits. | [25] |
| Sam Wise | 2B | 1890 | Wise had a .293 batting average in 119 games for Buffalo. | [26] |