| Name | Political party | County | Dates served |
| Samuel Powel | Federalist | Delaware, Philadelphia County | 1792–1793 |
| Anthony Morris | Federalist | Philadelphia County | 1793–1794 |
| William Bingham | Federalist | Delaware, Philadelphia County | 1794–1795 |
| Robert Hare Sr. | Federalist | Delaware, Philadelphia County | 1795–1799 |
| John Woods | Federalist | Allegheny, Washington County | 1799–1801 |
| Samuel Maclay | Democratic Republican | Northumberland County | 1801–1802 |
| John Pearson | Democratic Republican | Lancaster County | 1802–1803 |
| Robert Whitehill | Democratic Republican | Cumberland County | 1803–1805 |
| James Young Brady | Constitutionalist[a] | Armstrong, Indiana, Jefferson, Westmoreland County | 1805–1806 |
| John Francis Steele | Democratic Republican | Lancaster County | 1805–1806 |
| Presely Carr Lane Sr. | Democratic Republican | Fayette, Greene, Westmoreland County | 1806–1814 |
| Walter Lowrie | Democratic Republican | Allegheny, Beaver, Butler County | 1813–1814 |
| John Tod | Democratic Republican | Bedford, Cambria, Somerset County | 1814–1816 |
| Isaac Weaver Jr. | Democratic Republican | Greene, Washington County | 1817–1820 |
| William Marks | Democratic | Allegheny, Armstrong, Beaver, Butler County | 1820–1825 |
| Philip Swenk Markley | Democratic | Montgomery County | 1821–1822 |
| Thomas Burnside Sr. | Democratic | Centre, Clearfield, McKean, Potter, Tioga County | 1825–1826 |
| Alexander Mahon Sr. | Democratic | Cumberland, Perry County | 1825–1828 |
| William George Hawkins Sr. | Democratic | Fayette, Greene, Washington County | 1831–1832 |
| Thomas Ringland | Democratic | Greene, Washington County | 1833–1834 |
| Jesse Reading Burden | Democratic | Philadelphia County | 1833–1834 |
| Thomas Scott Cunningham | Democratic | Crawford, Erie, Mercer County | 1835–1836 |
| Jesse Reading Burden | Whig | Philadelphia County | 1837–1838 |
| Charles Bingham Penrose | Whig | Adams, Cumberland, Franklin, Perry, Philadelphia County | 1838–1839 |
| William Tennet Rogers | Democratic | Bucks County | 1839 |
| Ebeneezer Kingsbury Jr. | Democratic | Luzerne, Monroe, Pike, Wayne County | 1841 |
| John Strohm | Anti-Masonic | Lancaster, York County | 1841 |
| Charles Bingham Penrose | Whig | Adams, Cumberland, Franklin, Perry, Philadelphia County | 1841 |
| John Hoge Ewing | Whig | Washington County | 1842 |
| William Hiester | Whig | Lancaster, York County | 1842 |
| Benjamin Crispin | Democratic | Philadelphia County | 1843 |
| William Bigler | Democratic | Armstrong, Camrbia, Clarion, Clearfield, Indiana County | 1844–1845 |
| John Benton Sterigere | Democratic | Chester, Delaware, Montgomery County | 1845 |
| William Pendleton Wilcox | Democratic | Jefferson, McKean, Potter, Tioga, Venango, Warren County | 1845 |
| Daniel Lee Sherwood Jr. | Democratic | Tioga, Bradford County | 1846 |
| Charles L. Gibbons Sr. | Whig | Philadelphia County | 1847 |
| William Sterling Ross | Improvement Democrat[b] | Columbia, Luzerne County | 1847 |
| William Williamson Jr. | Whig | Chester, Delaware County | 1847 |
| William Freame Johnston | Whig | Chester, Cambria, Clearfield, Indiana County | 1848 |
| George Darsie | Whig | Allegheny, Butler County | 1848–1849 |
| Valentine Best | Democratic | Columbia, Luzerne County | 1849 |
| Benjamin Matthias | Whig | Philadelphia County | 1851 |
| Christan Myers | Whig | Armstrong, Clarion, Indiana County | 1852 |
| John Hoge Walker | Whig | Crawford, Erie County | 1852 |
| Thomas Erskin Carson | Whig | Adams, Franklin County | 1853 |
| John Christian Kunkel | Whig | Dauphin, Northumberland County | 1853 |
| Byron Delano Hamlin | Democrat | Clearfield, Elk, Forest, McKean,Potter, Tioga County | 1855 |
| William Muhlenberg Hiester | Democrat | Berks County | 1855 |
| William McKinney Piatt | Democrat | Bradford, Susquehanna, Wyoming County | 1855–1856 |
| Nathaniel Borrodaille Browne | Democrat | Philadelphia County | 1856 |
| Darwin Abel Finney | Whig | Crawford, Erie County | 1857 |
| David Taggart | Whig | Dauphin, Northumberland County | 1857 |
| John M. Cresswell Jr. | Democratic | Blair, Cambria, Clearfield, Huntingdon County | 1858 |
| William Henry Welsh | Democratic | York County | 1858 |
| Jacob Turney | Democratic | Fayette, Westmoreland County | 1859 |
| William Miller Francis | Republican | Lawrence, Mercer, Venango County | 1860 |
| Robert Moffett Palmer | Republican | Schuylkill County | 1860–1861 |
| Louis Williams Hall Sr. | Republican | Blair, Cambria, Centre, Clearfield, Huntingdon, Juniata, Mifflin, Perry County | 1861–1862 |
| George van Eman Lawrence | Republican | Beaver, Greene, Washington County | 1863 |
| John P. Penny Sr. | Republican | Allegheny County | 1863–1864 |
| Louis Williams Hall Sr. | Republican | Blair, Cambria, Centre, Clearfield, Huntingdon, Juniata, Mifflin, Perry County | 1864 |
| William J. Turrell | Republican | Bradford, Sullivan, Susquehanna, Wyoming County | 1865 |
| David Fleming | Republican | Dauphin, Lebanon County | 1866 |
| Louis Williams Hall Sr. | Republican | Blair, Cambria, Centre, Clearfield, Huntingdon, Juniata, Mifflin, Perry County | 1866–1867 |
| James Leonard Graham | Republican | Allegheny County | 1868 |
| Wilmer Worthington | Republican | Chester, Delaware, Montgomery County | 1869 |
| Charles Henderson Stinson | Republican | Chester, Delaware, Montgomery County | 1869–1870 |
| Albert Gallatin Brodhead | Democratic | Carbon, Luzerne, Monroe, Pike, Wayne County | 1871 |
| William Andrew Wallace | Democratic | Blair, Cambria, Cameron, Clarion, Clearfield, Clinton, Elk County | 1871 |
| James Smith Rutan | Republican | Allegheny, Beaver, Butler, Washington County | 1872 |
| George Holmes Anderson | Republican | Allegheny County | 1873 |
| George H. Cutler | Republican | Erie, Warren County | 1874 |
| Butler B. Strang | Republican | Cameron, McKean, Potter, Tioga County | 1874 |
| Henry Lloyd White | Republican | Armstorong, Cambria, Indiana, Jefferson, Westmoreland County | 1874 |
| Elisha W. Davis | Republican | Philadelphia County | 1875–1876 |