| Name | Institution | Year |
| John Abelson | California Institute of Technology | 1985 |
| Julius Adler | University of Wisconsin–Madison | 1978 |
| Paul Ahlquist | University of Wisconsin–Madison | 1993 |
| Bruce Alberts | University of California, San Francisco | 1981 |
| Jorge Allende | University of Chile | 2001 |
| Sidney Altman | Yale University | 1990 |
| Bruce Ames | University of California, Berkeley | 1972 |
| Gilbert Ashwell | National Institutes of Health | 1979 |
| Tania Baker | Massachusetts Institute of Technology | 2007 |
| Clinton Ballou (d) | University of California, Berkeley | 1975 |
| David Bartel | Massachusetts Institute of Technology | 2011 |
| Jacqueline Barton | California Institute of Technology | 2002 |
| Brenda Bass | University of Utah | 2015 |
| Philip Beachy | Johns Hopkins University | 2002 |
| Lorena Beese | Duke University | 2009 |
| Marlene Belfort | New York State Department of Health | 1999 |
| James Berger | Johns Hopkins University | 2013 |
| Klaus Biemann | Massachusetts Institute of Technology | 1993 |
| Pamela Björkman | California Institute of Technology | 2001 |
| Elizabeth Blackburn | University of California, San Francisco | 1993 |
| Elkan Blout (d) | Harvard University | 1969 |
| Michael Botchan | UC Berkeley | 2008 |
| Herbert Boyer | University of California, San Francisco | 1985 |
| Paul D. Boyer (d) | University of California, Los Angeles | 1970 |
| Roscoe Brady (d) | National Institutes of Health | 1975 |
| Ronald Breaker | Yale University | 2013 |
| Patrick O. Brown | Stanford University | 2002 |
| John Buchanan (d) | Massachusetts Institute of Technology | 1962 |
| Giulio Cantoni (d) | National Institutes of Health | 1983 |
| John Carbon | University of California, Santa Barbara | 1986 |
| H. E. Carter (d) | University of Arizona | 1953 |
| Thomas R. Cech | Howard Hughes Medical Institute | 1987 |
| Michael Chamberlin | University of California, Berkeley | 1986 |
| Britton Chance (d) | University of Pennsylvania | 1954 |
| Aaron Ciechanover | Technion-Israel Institute of Technology | 2007 |
| W. Wallace Cleland (d) | University of Wisconsin–Madison | 1985 |
| Philip Cohen | University of Dundee | 2008 |
| Seymour Cohen (d) | Marine Biological Laboratory | 1967 |
| Mildred Cohn (d) | University of Pennsylvania | 1971 |
| Joan W. Conaway | Stowers Institute for Medical Research | 2020 |
| Minor Coon (d) | University of Michigan | 1983 |
| Nicholas R. Cozzarelli (d) | University of California, Berkeley | 1989 |
| Nancy Craig | Johns Hopkins University | 2010 |
| Benjamin Cravatt III | Scripps Research Institute | 2014 |
| James Dahlberg | University of Wisconsin–Madison | 1996 |
| Seth Darst | Rockefeller University | 2008 |
| Earl Davie (d) | University of Washington | 1980 |
| Christian de Duve (d) | Christian de Duve Institute of Cellular Pathology | 1975 |
| Hector DeLuca | University of Wisconsin–Madison | 1979 |
| Peter Dervan | California Institute of Technology | 1986 |
| Raymond Deshaies | Caltech | 2016 |
| Jack Dixon | Howard Hughes Medical Institute | 2000 |
| Russell Doolittle | University of California, San Diego | 1984 |
| Jennifer Doudna | University of California, Berkeley | 2002 |
| Gideon Dreyfuss | University of Pennsylvania | 2012 |
| Gerald Edelman (d) | The Scripps Research Institute | 1969 |
| Ronald Estabrook | University of Texas Southwestern Medical Center at Dallas | 1979 |
| Gary Felsenfeld | National Institutes of Health | 1976 |
| Edmond Fischer (d) | University of Washington | 1973 |
| Perry A. Frey | University of Wisconsin–Madison | 1998 |
| Irwin Fridovich | Duke University | 1981 |
| E. Peter Geiduschek | University of California, San Diego | 1974 |
| Quentin Gibson (d) | Rice University | 1982 |
| Alexander Glazer | University of California System | 2001 |
| Rachel Green | Johns Hopkins University | 2012 |
| Carol Greider | Johns Hopkins University | 2003 |
| Marianne Grunberg-Manago | Institut de Biologie Physico-Chimique | 1982 |
| Michael Grunstein | University of California, Los Angeles | 2008 |
| Irwin Gunsalus (d) | University of Illinois at Urbana–Champaign | 1965 |
| Lowell Hager | University of Illinois at Urbana–Champaign | 1995 |
| Sen-itiroh Hakomori | Pacific Northwest Research Institute | 2000 |
| Gordon Hammes (d) | Duke University | 1973 |
| Gregory Hannon | Cold Spring Harbor Laboratory | 2012 |
| F. Ulrich Hartl | Max Planck Institute of Biochemistry | 2011 |
| Ari Helenius | ETH Zurich | 2009 |
| Richard Henderson | Medical Research Council | 1998 |
| Wayne Hendrickson | Columbia University | 1993 |
| Leon Heppel (d) | Cornell University | 1970 |
| Avram Hershko | Technion Israel Institute of Technology | 2003 |
| George Hess | Cornell University | 1987 |
| Robert L. Hill | Duke University | 1975 |
| Mahlon Hoagland (d) | Worcester Foundation for Biomedical Research | 1984 |
| B. L. Horecker (d) | Cornell University | 1961 |
| Arthur Horwich | Yale University | 2003 |
| Robert Huber | Max Planck Institute for Biochemistry | 1995 |
| Jerard Hurwitz (d) | Memorial Sloan-Kettering Cancer Center | 1974 |
| Clyde Hutchison | University of North Carolina at Chapel Hill | 1995 |
| William Jencks (d) | Brandeis University | 1971 |
| Gerald Joyce | Scripps Research Institute | 2001 |
| Howard Ronald Kaback | University of California, Los Angeles | 1987 |
| Paul Kaesberg (d) | University of Wisconsin–Madison | 1991 |
| Seymour Kaufman (d) | National Institutes of Health | 1986 |
| Yoshito Kaziro (d) | Kyoto University | 2000 |
| Eugene P. Kennedy (d) | Harvard University | 1964 |
| Peter Kim | Merck & Co., Inc. | 1997 |
| Robert Kingston | Harvard University | 2016 |
| Judith Klinman | University of California, Berkeley | 1994 |
| Jeremy Knowles (d) | Harvard University | 1988 |
| Edward Korn | National Institutes of Health | 1990 |
| Arthur Kornberg (d) | Stanford University | 1957 |
| Roger Kornberg | Stanford University | 1993 |
| Alberto Kornblihtt | University of Buenos Aires | 2011 |
| Daniel E. Koshland, Jr. (d) | University of California, Berkeley | 1966 |
| Stephen Kowalczykowski | University of California, Davis | 2007 |
| Joseph Kraut (d) | University of California, San Diego | 1988 |
| John Kuriyan | University of California, Berkeley | 2001 |
| Alan Lambowitz | University of Texas at Austin | 2004 |
| Arthur Landy | Brown University | 1999 |
| M. Daniel Lane | Johns Hopkins University | 1987 |
| Henry Lardy (d) | University of Wisconsin–Madison | 1958 |
| I. Robert Lehman | Stanford University | 1977 |
| William Lennarz | State University of New York at Stony Brook | 1989 |
| Christopher D. Lima | Memorial Sloan Kettering Cancer Center | 2020 |
| Laszlo Lorand (d) | Northwestern University | 1987 |
| George Lorimer | University of Maryland, College Park | 1997 |
| James Manley | Columbia University | 2011 |
| Lynne E. Maquat | University of Rochester | 2011 |
| Michael Marletta | The Scripps Research Institute | 2006 |
| Emanuel Margoliash (d) | Northwestern University | 1975 |
| Brian Matthews | University of Oregon | 1986 |
| Rowena Green Matthews | University of Michigan | 2002 |
| Stephen L. Mayo | California Institute of Technology | 2004 |
| Robert Bruce Merrifield (d) | Rockefeller University | 1972 |
| Stanley Miller (d) | University of California, San Diego | 1973 |
| Kiyoshi Mizuuchi | National Institutes of Health | 1994 |
| Paul Modrich | Duke University | 1993 |
| Elizabeth Neufeld | University of California, Los Angeles | 1977 |
| Marshall Warren Nirenberg (d) | National Institutes of Health | 1967 |
| Harry Noller | University of California, Santa Cruz | 1992 |
| Masayasu Nomura (d) | University of California, Irvine | 1978 |
| Peter Novick | University of California, San Diego | 2013 |
| Michael E. O'Donnell | The Rockefeller University | 2006 |
| Erin O'Shea | University of California, San Francisco | 2004 |
| Leslie Orgel (d) | Salk Institute for Biological Studies | 1990 |
| Roy Parker | University of Colorado Boulder | 2012 |
| Armando Parodi | Fundacion Instituto Leloir | 2000 |
| Dinshaw Patel | Memorial Sloan-Kettering Cancer Center | 2009 |
| Nikola Pavletich | Memorial Sloan-Kettering Cancer Center | 2012 |
| David Phillips (d) | University of Oxford | 1985 |
| Frank Putnam (d) | Indiana University | 1976 |
| Charles Radding | Yale University | 1995 |
| Venkatraman Ramakrishnan | Medical Research Council | 2004 |
| Linda Randall | University of Missouri | 1997 |
| Tom Rapoport | Harvard University | 2005 |
| Lester Reed (d) | University of Texas at Austin | 1973 |
| Douglas C. Rees | California Institute of Technology | 2000 |
| Peter Reichard | Karolinska Institute | 1980 |
| Danny Reinberg | New York University | 2015 |
| Alexander Rich (d) | Massachusetts Institute of Technology | 1970 |
| Charles C. Richardson | Harvard University | 1983 |
| Phillips Robbins | Boston University | 1982 |
| John D. Roberts (d) | California Institute of Technology | 1956 |
| Ellen Rothenberg | California Institute of Technology | 2021 |
| Saul Roseman (d) | Johns Hopkins University | 1972 |
| William J. Rutter | Synergenics, LLC | 1984 |
| Bengt I. Samuelsson | Karolinska Institute | 1984 |
| Anthony San Pietro (d) | Indiana University | 1983 |
| Aziz Sancar | University of North Carolina at Chapel Hill | 2005 |
| Frederick Sanger (d) | Medical Research Council | 1967 |
| Robert Sauer | Massachusetts Institute of Technology | 1996 |
| Howard Schachman (d) | University of California, Berkeley | 1968 |
| Gottfried Schatz | University of Basel | 1989 |
| Robert Schimke (d) | Stanford University | 1976 |
| Paul Schimmel | Scripps Research Institute | 1990 |
| Vern Schramm | Albert Einstein College of Medicine | 2007 |
| Brenda Schulman | St. Jude Children's Research Hospital | 2014 |
| Kevan Shokat | University of California, San Francisco | 2009 |
| Maxine Singer | Carnegie Institution of Washington | 1979 |
| Robert Sinsheimer | University of California, Santa Barbara | 1967 |
| Emil Smith (d) | University of California, Los Angeles | 1962 |
| Hamilton O. Smith | J. Craig Venter Institute | 1980 |
| Dieter Söll | Yale University | 1997 |
| Nahum Sonenberg | McGill University | 2015 |
| David Sprinson (d) | Columbia University | 1990 |
| E. R. Stadtman (d) | National Institutes of Health | 1969 |
| Thressa Stadtman (d) | National Institutes of Health | 1981 |
| George Stark | Cleveland Clinic Foundation | 1987 |
| Donald Steiner (d) | University of Chicago | 1975 |
| Joan A. Steitz | Yale University | 1983 |
| Thomas Steitz (d) | Yale University | 1990 |
| Audrey Stevens (d) | Oak Ridge National Laboratory | 1998 |
| Bruce Stillman | Cold Spring Harbor Laboratory | 2000 |
| Jack Strominger | Harvard University | 1970 |
| JoAnne Stubbe | Massachusetts Institute of Technology | 1992 |
| Julian M. Sturtevant (d) | Yale University | 1973 |
| Wesley Sundquist | University of Utah | 2014 |
| Jack Szostak | Harvard University | 1998 |
| Herbert Tabor (d) | National Institutes of Health | 1977 |
| Paul Talalay (d) | Johns Hopkins University | 1987 |
| Glauco Tocchini-Valentini | National Research Council of Italy | 2009 |
| Susan S. Taylor | University of California, San Diego | 1996 |
| Jeremy Thorner | UC Berkeley | 2015 |
| Robert Tjian | University of California, Berkeley | 1991 |
| Olke Uhlenbeck | Northwestern University | 1993 |
| P. Roy Vagelos | Merck & Co., Inc. | 1972 |
| Bert Vallee (d) | Harvard University | 1974 |
| Alexander Varshavsky | California Institute of Technology | 1995 |
| Sidney Velick (d) | University of Utah | 1981 |
| Peter von Hippel | University of Oregon | 1978 |
| Salih Wakil (d) | Baylor College of Medicine | 1990 |
| Christopher T. Walsh | Harvard University | 1989 |
| Peter Walter | University of California, San Francisco | 2004 |
| James C. Wang | Harvard University | 1986 |
| Herbert Weissbach | Florida Atlantic University | 1982 |
| Jonathan Weissman | University of California, San Francisco | 2009 |
| James A. Wells | University of California, San Francisco | 1999 |
| Stephen C. West | Francis Crick Institute | 2016 |
| Reed Wickner | National Institutes of Health | 2000 |
| Sue Hengren Wickner | National Institutes of Health | 2004 |
| William T. Wickner | Dartmouth College | 1996 |
| Richard Wolfenden | University of North Carolina at Chapel Hill | 2002 |
| Carl Wu | National Institutes of Health | 2006 |
| Wei Yang | National Institutes of Health | 2013 |
| Paul Zamecnik (d) | Harvard University | 1968 |